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BIG CHAP(Detergent zwitterionic) S114 [N,N'-Bis(3-D-gluconamidopropyl)cholamide], 99+%[ 86303-22-2 ] f.w.: 878.1 daltons |
BIG CHAP is a non-ionic detergent possessing deoxycholic acid and a gluconamide polar group. It has a high CMC value of 2.9 mM and can be easily separated by dialysis. Because its UV absorbance is low, it can be used for the determination of proteins.BIG CHAP has been used for the extraction of opioid receptors from neuroblastoma or hybrid cells of glyoma. It has also been applied to adenylate cyclase or acetyltransferase. These detergents are also widely used to solubilize chromophores or to stabilize enzymes in diagnostic analyses and biochemical assays.
| Quantity | Price | |
|---|---|---|
| 1gm | $88.00 |  |
| 2gm + | $167.00 | |
| 5gm + | $417.00 | |
| 10gm + | $574.00 | |
| Download***Certificate of Analysis*** | ||
Non ionic detergent analog of CHAPS and CHAPSO with significantly reduced electrostatic interactions that do not interfere in anion exchange chromotography. Purity > 95% by TLC. Soluble in water.
- L.M. Hjelmeland, L.M. Klee, W.A. and Osbourne, J.C., Anal. Biochem, 130, 485 (1983).
- CMC (H2O): ~ 2.9 mM (0.25%)
- Aggregation Number (H2O): ~ 10
- Purity: ? 95% pure by HPLC analysis.
- pH (1% solution): 5-8
- Solubility in water at 20°C: 10%
- Conductance (10% solution): < 100 S
- Percent fluorescence due to a 0.1% detergent solution at 345 nm: < 10
- Absorbance of a 1% detergent solution: 340 nm: < 0.05 280 nm: < 0.30 260 nm: < 0.40