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BIOTIN HPDP(Biotinylation Reagent) B106 N-[6-(Biotinamido)hexyl]-3-(2-pyridyldithio)propionamidef.w.: 539.8 daltons |
HPDP biotin is a pyridyl dithiol biotin compound for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in near neutral buffers to form reversible disulfide bonds. Biotinylation occurs between pH 6-9. The pyridine-2-thione leaving group can be measured at 343nm to determine the degree of biotinylation.
| Quantity | Price | |
|---|---|---|
| 50mgm + | $256.00 |  |
| 100mgm + | $483.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 29.2 angstroms. Sulfhydryl reactive biotinylating reagent. Biotinylation occurs between pH 6-9. Pyridyl dithiol reacts with sulfhydryl groups to form a stable sulfhydryl bond. The pyridine-2-thione leaving group can be measured at 343nm to determine the degree of biotinylation. Purity >94% by HPLC.
- Ghebrihiwet, B., et al, J. Immunol. Methods, 110, 251-260 (1988).
- Ishmael, F.T., et al, J. Biol. Chem., 276, 25236-25242 (2001).
- Slatin, S.L., et al, Proc. Natl. Acad. Sci. USA, 99, 1286-1291 (2002).
- Biotin HPDP biotinylates antibodies or other proteins for use in protein methods
- Thiol reactive - Biotin HPDP reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
- Pyridyldithiol activated perform reactions at pH 6.5 to 7.5 in buffers such as PBS
- The reaction is reversible and forms disulfide bonds, which can be cleaved using DTT, TCEP, DTBA or other reducing agent
- Must be dissolved in DMSO or DMF before further dilution in aqueous buffers
- Medium length spacer arm is 29.2 angstroms