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BIOTIN HYDRAZIDE(Biotinylation Reagent) B108 Biotin hydrazide[ 66640-86-6 ] f.w.: 258.3 daltons |
Hydrazide biotin is a short length, hydrazide activated biotinylation reagent for labeling glycoproteins and other carbohydrate containing compounds having oxidizable sugars or aldehydes.
| Quantity | Price | |
|---|---|---|
| 100mgm + | $113.00 |  |
| 250mgm + | $267.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 15.7 angstroms. Carbohydrate reactive biotinylation reagent. Biotinylates immunoglobulins without loss of immunological activity. Use at pH 4-6 to complete coupling reaction. Purity >95% by TLC.
- O\'Shannessey, D.J., et al. (1984) A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties. Immunol. Lett. 8, 273-277.
- Kähne, T. and Ansorge, S. (1994) Non-radioactive labeling and immunoprecipitation analysis of leukocyte surface proteins using different methods of protein biotinylation. J. Immunol. Methods 168, 209-218.
- Bayer, E.A., et al. (1988) Biocytin hydrazide-a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. Anal. Biochem. 170, 271-281.O’Shannessey, D.J. and Quarles, R.H. (1987) Labeling of the oligosaccharide moieties of immunoglobulins. J. Immunol. Meth. 99, 153-161.
- O\'Shannessy, D. J. and Quarles R.H. (1985) Specific conjugation reactions of the oligosaccharide moieties of immunoglobulins. J. Appl. Biochem. 7, 347-355.
- O\'Shannessey, D.J., et al. (1987) Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Anal. Biochem. 163, 204-209.
- Reisfield, A., et al. (1987) Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem. Biophys. Res. Com. 142, 519-526.
- Rosenberg , M.B., et al. (1986) Receptor binding activities of biotinylated derivatives of ß-nerve growth factor. J. Neurochem. 46, 641-648.
- Scott, M.G., et al. (2000) Cationic Antimicrobial Peptides Block the Binding of Lipopolysaccharide (LPS) to LPS Binding Protein. J. Immunol. 164, 549-553.
- Wade, D.P., et al. (1985) Detection of the low density-lipoprotein receptor with biotin-low density lipoprotein. Biochem. J. 229, 785-790.
- Biotin hydrazide biotinylates glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins
- Biotinylate and isolate cell surface glycoproteins
- Aldehyde reactive reacts with aldehydes formed by periodate oxidation of sugar groups
- Hydrazide activated perform reactions at pH 4 to 6 in buffers such as sodium acetate
- Biotin hydrazide is irreversible and forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved
- Solubility-usually dissolved in DMSO before further dilution in aqueous buffers
- Spacer arm 15.7 angstroms
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