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EMCS(Protein Crosslinker heterobifunctional) CL222 N-(epsilon-Maleimidocaproyloxy)succinimide[ 55750-63-5 ] f.w.: 308.3 daltons |
EMCS is an amine-to-sulfhydryl crosslinker that contains NHS ester and maleimide reactive groups at opposite ends of a medium length spacer arm (9.4 angstroms).
| Quantity | Price | |
|---|---|---|
| 50mgm + | $196.00 |  |
| 100mgm + | $370.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 9.4 angstroms. Heterobifunctional crosslinker. Sulfhydryl and amine reactive reagent used for enzyme immunoassay. Non-cleavable, water insoluble. Aliphatic spacer offers low potential for eliciting an immune response.
- Fujiwara, K., et al. (1988). J. Immunol. Methods 112, 77-83.
- Peeters, J.M., et al. (1989). J. Immunol. Methods 120, 133-143.
- Hermanson, G.T. (1996). Bioconjugate Techniques, San Diego: Academic Press, pp. 243-245.
- NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
- Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
- Non-cleavable, water-insoluble
- Increased sphere of coupling vs. GMBS
- Aliphatic spacer offers low potential for eliciting an immune response
- Reactive groups: maleimide and NHS ester
- Reactive toward: sulfhydryl and amino groups