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IODOACETYL LC BIOTIN(Biotinylation Reagent) B107 N-Iodoacetyl-N-biotinylhexylenediaminef.w.: 510.4 daltons |
Iodoacetyl LC Biotin is a medium length, haloacetyl activated, sulfhydryl reactive biotinylation reagent that forms stable, irreversible thioether bonds at alkaline pH.
| Quantity | Price | |
|---|---|---|
| 50mgm + | $185.00 |  |
| 100mgm + | $350.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 27.1 angstroms. Biotinylation reagent. Reacts specifically with thiol groups at pH 7.5-8.5 to form stable thiol-ether bond. Reagent is not water soluble so a small amount of DMSO or DMF should be added to the ligand containing buffer. Soluble in DMSO and DMF.
- Sutoh, K., Yamamoto, K. and Wakabayashi, T. (1984). J. Mol. Biol. 178, 323-339.
- Yamamoto, K., Sekine, T. and Sutoh, K. (1984). FEBS 176, 75-78.
- Iodoacetyl LC hydrazide biotinylates antibodies or other proteins for use in protein methods
- It is membrane permeable and can be used to label inside cells (intracellular)
- Thiol reactive - Iodoacetyl LC biotin reacts with sulfhydryls (-SH), such as the side-chain of cysteine
- Iodoacetyl activated It can perform reactions in the dark at pH 7.5 to 8.5 in Tris or borate buffer
- Iodoacetyl biotin is irreversible and forms permanent thioether bonds; spacer arm cannot be cleaved
- Must be dissolved in DMSO or DMF before further dilution in aqueous buffers
- Medium length spacer arm (27.1 angstroms). Iodoacetyl LC biotin contains a hexylenediamine extension.