BCIP -t 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
| Catalog # | BR100 |
|---|---|
| CAS Number | 6578-06-9 |
| Formula Weight | f.w.: 433.6 daltons |
| Source | Yacoo |
BCIP-T (5-bromo-4-chloro-3-indolyl phosphate, p-toluidine salt) is a chromogenic substrate for alkaline phosphatase used in conjunction with the oxidant NBT (nitro blue tetrazolium) to enhance blue color development. Alkaline phosphatase catalyses the removal of a phosphate group from BCIP-T, generating a product that oxidizes and dimerizes to dibromodichloro indigo. The reducing equivalents produced during the dimerization reaction reduce NBT to an insoluble purple dye, diformazan.
Pricing & Ordering
| Quantity | Price | Action |
|---|---|---|
| 1gm | $95.00 |
|
| 5gm + | $470.00 |
|
| 500mgm + | $59.00 |
|
Preparation of a 50 mg/mL stock solution in dimethylformamide
1.J. Sambrook, D. W. Russell, Molecular Cloning: A Laboratory Manual, the Third edition (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 16.42, 2001).