NHS BIOTIN N-Hydroxysuccinimidobiotin

Catalog #	B101
CAS Number	35013-72-0
Molecular Formula	C14H19N3O5S
Formula Weight	f.w.: 341.4 daltons

NHS Biotin is the simplest NHS ester activated biotinylation reagent for labeling primary amines such as protein lysines, The short spacer arm of NHS biotin allows membrane permeability. This enables NHS biotin to be widely used for general intracellular labeling.

Pricing & Ordering

Quantity	Price	Action
100mgm +	$101.00	Quantity
250mgm +	$333.00	Quantity

Product Documents

Certificate of Analysis

Spacer arm 13.5 angstroms. Biotinylation reagent. Biotinylates amino acids, peptides or proteins under mild conditions. Purity>95% by TLC. Soluble in DMSO.


Flanders, K.C., et al, Biochemistry, 21, 4244-4251 (1982). 
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Wojchowski, D.M., Sytkowski, A.J., J. Immunol. Methods, 90, 173-177 (1986). 
Nunomura, W., et al, J. Biol. Chem., 275, 6360-6367 (2000). 
Nakayama, H., et al, J. Biol. Chem., 265(24), 14335-14357 (1990). 
Nunomura, W., et al, J. Biol. Chem., 275(9), 6360-6367 (2000).


NHS biotin biotinylates, amino acids, peptides or proteins under mild conditions 
Penetrates cell membranes and can be used to label inside cells 
Soluble in DMSO and DMF. Dissolve in DMSO or DMF prior to dilution in aqueous systems 
NHS biotin is used to biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resin 
Forms stable amide bonds therefore the reaction is not reversable

N-Hydroxysuccinimidobiotin (NHS BIOTIN): Product Spotlight

A focused product spotlight on N-Hydroxysuccinimidobiotin, product code NHS BIOTIN, covering its confirmed identity, role in biotinylation workflows, common…