Biotin hydrazide

Catalog #	B108
CAS Number	66640-86-6
Molecular Formula	C10H18O2N4S
Formula Weight	f.w.: 258.3 daltons

Hydrazide biotin is a short length, hydrazide activated biotinylation reagent for labeling glycoproteins and other carbohydrate containing compounds having oxidizable sugars or aldehydes.

Pricing & Ordering

Quantity	Price	Action
100mgm +	$113.00	Quantity
250mgm +	$267.00	Quantity

Product Documents

Certificate of Analysis

Spacer arm 15.7 angstroms. Carbohydrate reactive biotinylation reagent. Biotinylates immunoglobulins without loss of immunological activity. Use at pH 4-6 to complete coupling reaction. Purity >95% by TLC.


O\'Shannessey, D.J., et al. (1984) A novel procedure for labeling immunoglobulins by conjugation to oligosaccharide moieties. Immunol. Lett. 8, 273-277. 
KÃ¤hne, T. and Ansorge, S. (1994) Non-radioactive labeling and immunoprecipitation analysis of leukocyte surface proteins using different methods of protein biotinylation. J. Immunol. Methods 168, 209-218. 
Bayer, E.A., et al. (1988) Biocytin hydrazide-a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. Anal. Biochem. 170, 271-281.Oâ€™Shannessey, D.J. and Quarles, R.H. (1987) Labeling of the oligosaccharide moieties of immunoglobulins. J. Immunol. Meth. 99, 153-161. 
O\'Shannessy, D. J. and Quarles R.H. (1985) Specific conjugation reactions of the oligosaccharide moieties of immunoglobulins. J. Appl. Biochem. 7, 347-355. 
O\'Shannessey, D.J., et al. (1987) Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Anal. Biochem. 163, 204-209. 
Reisfield, A., et al. (1987) Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem. Biophys. Res. Com. 142, 519-526. 
Rosenberg , M.B., et al. (1986) Receptor binding activities of biotinylated derivatives of ÃŸ-nerve growth factor. J. Neurochem. 46, 641-648. 
Scott, M.G., et al. (2000) Cationic Antimicrobial Peptides Block the Binding of Lipopolysaccharide (LPS) to LPS Binding Protein. J. Immunol. 164, 549-553. 
Wade, D.P., et al. (1985) Detection of the low density-lipoprotein receptor with biotin-low density lipoprotein. Biochem. J. 229, 785-790.


Biotin hydrazide biotinylates glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins 
Biotinylate and isolate cell surface glycoproteins
Aldehyde reactive reacts with aldehydes formed by periodate oxidation of sugar groups 
Hydrazide activated perform reactions at pH 4 to 6 in buffers such as sodium acetate 
Biotin hydrazide is irreversible and forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved 
Solubility-usually dissolved in DMSO before further dilution in aqueous buffers 
Spacer arm 15.7 angstroms