Application Note: Using SAND and APG in Exploratory Photoreactive Protein Crosslinking Workflows
When researchers need to preserve short-lived protein associations long enough to compare conditions, a photoreactive workflow can be useful as an exploratory method. This application note shows how Sulfosuccinimidyl-2-(m-azido-o-nitrobenzamido)ethyl-1,3'-dithiopropionate (SAND) can be positioned as the primary reagent in that workflow, with p-Azidophenylglyoxal hydrate (APG) used as an example supporting reagent for comparison during method development.
Dr. Sarah Johnson
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