A focused look at Imidazole, 99+%, Molecular Biology Grade, including its chemistry, role in protein workflows, common applications, and core product specifications.
Chemistry and structure
Imidazole, 99+%, Molecular Biology Grade is identified by CAS 288-32-4 and the molecular formula C3H4N2. Chemically, imidazole is a five-membered heterocyclic ring containing two nitrogen atoms. That compact ring structure is the basis for its frequent use in laboratory workflows where a small nitrogen-containing organic compound is needed in solution preparation and protein-related methods.
For this product spotlight, the key point is product identity: Imidazole, 99+%, Molecular Biology Grade is supplied for molecular biology use and specified at 99+% purity. In practice, users typically select this reagent when they need a consistent imidazole source for laboratory protocols involving protein handling and purification-related buffer systems.
How it works
In protein workflows, imidazole is commonly associated with purification methods for histidine-tagged proteins. In that context, it is used in buffer systems to help manage interactions involved in immobilized metal affinity purification. Depending on the step in the workflow, imidazole may be present at lower levels to reduce nonspecific binding or at higher levels to support recovery of the target protein from the purification medium.
Because it is a defined small-molecule reagent, imidazole is often chosen when a protocol calls for controlled adjustment of purification conditions rather than a more complex additive. Its role is therefore practical and workflow-based: it helps support protein purification steps in methods where imidazole is part of the established buffer design.
Typical applications
- His-tagged protein purification: Used in buffer systems for workflows involving histidine-tagged recombinant proteins.
- Protein preparation protocols: Included where imidazole is specified as part of solution or buffer preparation.
- Molecular biology laboratory workflows: Selected when a molecular biology grade imidazole reagent is required for routine bench use.
- Method development: Useful in optimization of purification conditions that call for imidazole-containing buffers.
Specifications
| Property | Specification |
|---|---|
| Product name | Imidazole, 99+%, Molecular Biology Grade |
| Product code | IMIDAZOLE |
| CAS | 288-32-4 |
| Molecular formula | C3H4N2 |
| Grade | Molecular Biology Grade |
| Assay | 99+% |
Considerations
As with any laboratory reagent, imidazole should be handled according to the needs of the specific protocol and the applicable documentation for the material in use. Users should confirm that the selected grade, concentration range, and buffer composition are appropriate for their workflow before use.
In protein purification methods, imidazole levels can affect downstream processing, so it is important to align reagent use with the requirements of the next analytical or preparation step. Method compatibility, solution preparation accuracy, and protocol-specific handling should all be reviewed during process planning.
To review product details for Imidazole, 99+%, Molecular Biology Grade, visit the product page and confirm fit with your molecular biology workflow.