DTNB Ellman's Reagent 5,5'-Dithio-bis (2-nitrobenzoic acid)
| Catalog # | CS106 |
|---|---|
| CAS Number | 69-78-3 |
| Formula Weight | f.w.: 396.4 daltons |
DTNB, Ellman's reagent, is a versatile, water soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412 nanometers.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time. DTNB is a yellow crystalline powder and used for the total determination of thiol groups in biological samples.
Pricing & Ordering
| Quantity | Price | Action |
|---|---|---|
| 2gm + | $39.00 |
|
| 5gm + | $92.00 |
|
| 10gm + | $175.00 |
|
1.Ellman, G.L. (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70-7. 2.Riddles, P.W., et al. (1983) Reassessment of Ellman’s reagent. Meth Enzymol 91:49-60. 3.Riddles, P.W., et al. (1979) Ellman’s reagent: 5, 5'-dithiobis(2-nitrobenzoic acid) - a reexamination. Anal Biochem 94:75-81. 4.Kuwata, K., et al. (1982) Liquid chromatographic determination of alkylthiols via derivatization with 5, 5’- dithiobis(2-nitrobenzoic acid). Anal Chem 54:1082-7. 5.Masamune, S, et al. (1989) Bio-Claisen condensation catalyzed by thiolase from Zoogloea ramigera. Active site cysteine residues. Chemtracts-Organic Chem 2:247-51. 6.Tsukamoto, Y. and Wakil, S.J. (1988) Isolation and mapping of the beta-hydroxyacyl dehydratase activity of chicken liver fatty acid synthase. J Biol Chem 263:16225-9. 7.Alvear, M., et al. (1989) Fractionation of chicken liver mevalonate 5-diphosphate decarboxylase by sulfhydryl-directed reagents: evidence of a functional dithiol. Biochem Biophys Acta 994:7-11.
•Used for the quantificaion of sulfhydryls in biological samples •Water soluble •Store at 2-8C
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