DTSSP 3,3'-Dithiobis(sulfosuccinimidylpropionate)
Protein Crosslinker homobifunctional
| Catalog # | CL103 |
|---|---|
| CAS Number | 81069-02-5 |
| Molecular Formula | C14H12N2O14S4Na |
| Formula Weight | f.w.: 608.5 daltons |
DTSSP is a water soluble crosslinker that contains amine reactive NHS ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents such as DTT, TCEP or DTBA.
Pricing & Ordering
| Quantity | Price | Action |
|---|---|---|
| 50mgm + | $127.00 |
|
| 100mgm + | $240.00 |
|
Product Documents
Spacer arm 12.0 angstroms. Homobifunctional reagent. Thiol cleaveable crosslinker. The disulfide bond can be cleaved with 50mmoles DTT, 100mmoles 2-mercaptoethanol, or 1% sodium borohydride. Has been used to immobilize proteins onto supports containing amino groups. Purity >95% by HPLC.
- Back, J. W., et al. (2002). Protein Sci. 11, 2471-2478.
- Bennett, K.L., et al. (2000). Protein Sci. 9, 1503-1518.
- Cheng, S.K., et al. (2004) PloS Biology.2, e30.
- Paine-Saunders, S., et al. (2002) Heparan Sulfate Proteoglycans Retain Noggin at the Cell Surface. A Potential Mechanism for Shaping Bone Morphogenetic Protein Gradients.J. Biol. Chem.277, 2089-2096.
- Liliana Schaefer, L., et al. (2005) The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages. J. Clin. Invest.115, 2223-2233.
•Amine reactive sulfo NHS ester reacts rapidly with any primary amine containing molecule
•Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
•Spacer arm is also cleaved with 5% ?-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
•Cleavable crosslinker allows separation of crosslinked products
•DTSSP crosslinker is not membrane permeable, so it is used for cell surface crosslinking
Articles about DTSSP
Protein Crosslinker homobifunctional Buyer's Guide | Soltec Ventures
A practical overview of Protein Crosslinker homobifunctional reagents, including what they are, where they are used, the products in scope, and how to choose…