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TRAUT'S REAGENT(Protein Modifier) M100 2-Iminothiolane[ 4781-83-3 ] f.w.: 137.6 daltons |
Traut's Reagent (2-iminothiolane) is a small thiolation compound that reacts with primary amines, for instance lysine side chains, to add a small spacer arm (8.1 angstroms) terminated by a free sulfhydryl group.
| Quantity | Price | |
|---|---|---|
| 1gm | $116.00 |  |
| 2.5gm + | $280.00 | |
| 5gm + | $505.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 8.1 angstroms. Used to introduce sulfhydyl groups onto primary amines. 2-Iminothiolane is water soluble and reacts between pH 7-10.
1.Traut, R.R., et al. (1973). Methyl 4-mercaptobutyrimidate as a cleavable cross-linking reagent and its application to the Escherichia coli 30S ribosome. Biochem 12(17):3266-73. 2.Jue, R., et al. (1978). Addition of sulfhydryl groups to Escherichia coli ribosomes by protein modification with 2-iminothiolane (methyl 4- mercaptobutyrimidate). Biochem 17(25):5399-5405. 3.D'Oro, U., et al. (2002). Regulation of constitutive TCR internalization by the zeta-chain. J Immunol 169:6269-78. 4.Newton, D.L., et al. (2001). Potent and specific antitumor effects of an anti-CD22-targeted cytotoxic ribonuclease: potential for the treatment of non-Hodgkin lymphoma. Blood 97:528-35. 5.Stanisic, D.I., et al. (2003). Analysis of immunological nonresponsiveness to the 19-kilodalton fragment of merozoite surface protein 1 of Plasmodium yoelii. Infec Immunity 71(10):5700-13. 6.Ghosh. S.S., et al, Bioconjugate Chemistry, 1,71-76(1990).
- Easy one step conversion of amines to sulfhydryls
- Selective and spontaneous modification of primary amines at pH 7-10
- Traut's reagent incorporates a five-atom spacer arm to reduce steric hindrance
- Traut's reagent preserves the original positive charge to preserve protein solubility