CHAPS

(Detergent zwitterionic)

S112

(3-[3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, 99+%

CAS#: [ 75621-03-3 ]

Formula Weight: f.w.: 614.9 daltons

CHAPS is a zwitterionic detergent that solubilizes proteins in their native state. It has all the advantages of sulfobetaine containing detergents - hydrophobic, bile salt, and anionic detergents in a single molecule. Has a low molecular weight and high cellular micellar concentration. Product can be removed from protein solutions with a detergent removing gel or by dialysis.

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Pricing & Ordering

Quantity Price Action
2gm + $26.00
5gm + $59.00
10gm + $114.00
25gm + $203.00
100gm + $941.00
Product Documents
Certificate of Analysis


Zwitterionic detergent. Has all the advantages of sulfobetaine containing detergents-hydrophobic, bile salt, and anionic detergents in a single molecule. Has a low molecular weight and high cellular micellar concentration. Product can be removed from protein solutions with a detergent removing gel or by dialysis. Purity > 99% by HPLC. Conductivity < 25 micromhos in a 10% solution.

    

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    2. 

  2. W.F. Simonds, G. Koski, R.A. Streaty, L.M. Hjelmeland, W.A. Klee, Proc. Natl. Acad. Sci. USA, 77, 4623 (1980) 
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  3. Bitonti, A.J., et al, Biochemistry, 21, 3650 (1982) 
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  4. Rivnay, B., et al, Biochemistry, 21, 6922 (1982) 
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  5. Murayama, T., et al, Biomed. Res., 14, 71 (1993) 
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  6. Caglio, S., et al, Electrophoresis(Weinheim fed. Repub. Ger.), 15, 209 (1994) 
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  7. Hu. W., et al, Anal. Chim. Acta., 285, 335, (9194) 
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  8. Loo, R.R.O., et al, Protein Sci., 3, 1975 (1994) 
    9. 

  9. Lundberg, B.J., J. Pharm. Sci., 83, 72 (1994) 
    10. 

  10.  Radko, S.P., et al, Anal. Biochem., 223, 82 (1994) 
    11. 

  11. Wetlaufer, D. B., et al, Protein Sci, 4, 1535 (1995)
    12. 

    

  • CMC (H2O): ~ 8 mM (0.49%) 
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  • Aggregation Number (H2O): ~ 10dn/dc (H2O): 0.1323 mL/g 
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  • Purity:  98% pure by HPLC analysis. 
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  • pH (1% solution): 5-8 
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  • Solubility in water:  0.5M 
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  • Conductance (0.5 M solution): < 200 ?S 
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  • Absorbance of a 1% detergent solution: 340 nm: < 0.05 280 nm: < 0.1 260 nm: < 0.2
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