(Protein Modifier)

M112   (D,L)-1,4-Dithiothreitol, 99.5+%, Molecular Biology Grade
[ 27565-41-9 ]   f.w.: 154.3 daltons

Clelands reagent, also known as dithiothreitol or DTT is a water soluble protective reagent for sulfhydryl groups. It is capable of maintaining monothiols completely in the reduced state and reduces disulfudes quantitatively. Since its introduction in 1964 by Professor Cleland it has gained widespread acceptance as the reagent of choice for the protection of sulfhydryl groups. DTT readily permeates cell membranes. therefore DTT protects protein sulfhydryls allowing enzyme activity to be restored due to the oxidation of sulfhydryl groups in vitro. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N?-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

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m.p. 42-44C°; Protective reagent for sulfhydryl groups. Quantitatively reduces disulfides. The white crystals are clear and colorless in solution. Reagent promotes proteins to unfold and maximize bonding. 99% (titration) H2O: soluble50 mg/mL

  1. Cleland, W.W., Biochemistry, 3, 480-482 (1964) 
  2. Lauriault, V.V.M., OBrien, P.J., Mol. Pharmacol., 40, 125 (1991). 
  3. Klonne, D.R., Johnson, D.R., Toxicol. Lett., 42, 199 (1988).
  • DTT quantitatively reduces disulfides
  • Protective reagent for sulfhydryl groups
  • DTT promotes proteins to unfold and maximize bonding