TCEP Electrophoresis grade

TCEP HCl is further purifed for electrophoresis. This TCEP is autoclaved and SDS-PAGE tested. The product is comepletely RNAase free.Product is a superior alternative to DTT. The product is odorless, non-volatile and stable in air. Dilute solutions in air show no appreciable oxidation. Reduces organic disulfides to thiols rapidly and quantitatively in water. The reaction is reversible. Does not react with other functional groups on proteins.

K. C., Dixon, J.E., Anal. Biochem, 161, 524-528 (1987). 
Schonberg, A., Chem Ber., 163-164 (1935). 
Rauhut, M., et. al., JACS, 81, 1103-1107 (1959). 
Chen, C.S., Fujimoto, Y, girdoukas, G., Sih, C.J., JACS, 109, 6825-6309 (1987). 
Kirley, T.L., Anal. Biochem, 180, 231-236 (1989). 
Andrews, P C., Dixon, J.E., Anal. Biochem., 161, 524-528 (1987). 
Y., Girdoukas, G.Sih, C.J., JACS, 109, 6825-6309 (1987). 
Houk, J., Whitesides, G.M., JACS, 109, 6825-6836 (1987). 
Grayson, M., Farley, C.E., Chimie Organique du l du Centre Natl de la Recherche Scientifique, No. 182 CNRS, Paris, 275-284 (1970). 
Overman, L.E OConner, E.M., JACS, 98, 771-775 (1977). 
Rosenfield, R.E., Parthasarthy, R., Dunitis, J.D., JACS, 99, 4860-4862 (1977). 
Ruegg, U.T. and Rudinger, J., Methods Enzymology, 47, 111-126, (1977). 
Burns, J.A., et al, J. Org. Chem, 56, 2648-2650, (1991). 
Mery, J., et al,, Peptide Protein Research, 42, 44-52, (1993). 
Gray, W. R., Protein Science, 2, 1732-1748, (1993). 
Fisher, W.H., et al, Mass Spectrometry, 7, 225-228, (1993). 
Bieri, S, et al, Biochemistry, 34, 13059-13065, (1995). 
Tam, J., P,, et al, Proc. Natl Acad. Sci. USA, 92, 12485-12489, (1995). 
Blauenstein, P., et al, Eur. J. Nucl. Med., 22, 690-698 (1995). 
Gorman, J.J., et al, Rapid Communications Mass Spectrometry, 10, 529-536 (1996). 
Kiirsch, T., et al, Protein Expression Purification, 8, 75-84 (1996). 
Wu, J. and Watson, J.T., Protein Science, 6, 391-398 (1997). 
Bernard, C. L., et al, Exp. Brain Res., 113, 343-352 (1997). 
Huh, K. and Wenthold, R.J., Journal Biological Chemistry, 274, 151-151 (1999). 
Oda, Y. et al, Nature Biotech, 19, 379-382 (2001).

Odorless and non-volatile
Solid is stable in air for several months
Dilute TCEP solutions in air show no appreciable oxidation up to at least 72 hours pH<7.66
Solubility in water 310gm/liter
TCEP reduces organic disulfides to thiols rapidly and quantitatively in water
More complete reductions than 1,4-dithiothreitol ( DTT ), Clelands reagent
Even very stable alkyl disulfides are completely reduced at room temperature and pH 5 in less than 5 min.
Dilute (1mM) TCEP solutions react rapidly at room temperature
The strength of the phosphorus-oxygen bond makes the reaction irreversible
Kinetics rather than thermodynamics controls the reduction
Selectivity!
TCEP is selective for disulfides
TCEP does not react with other functional groups on proteins
Unreactive towards many common alkylating reagents so reductions have been carried out simultaneously with alkylations
Electrophoresis grade
SDS-PAGE tested
Autoclaved