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NHS LC BIOTIN(Biotinylation Reagent) B104 Succinimidyl-6-(biotinamido)hexanoate[ 72040-63-2 ] f.w.: 455.5 daltons |
NHS LC Biotin has an extended spacer arm and reduces steric hindrance when binding several biotinylated molecules to one avidin complex.
| Quantity | Price | |
|---|---|---|
| 50mgm + | $159.00 |  |
| 100mgm + | $300.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 22.4 angstroms. Biotinylation reagent. Reacts with primary amines to biotinylate proteins under mild conditions. Purity >94% by TLC. Soluble in DMSO.
- Burgess, T.L., et al, J. Biol. Chem., 270(32), 19188-19196 (1995).
- Gretch, D.R., et al, Anal. Biochem., 163, 270-277 (1987).
- Ying, S.C., et al, J. Immunol., 143(1), 221-228 (1989).
- Herwald, H., et al, J. Biol. Chem., 270(24), 14634-14642 (1995)
- Jans, D.A.., , et al, J. Biol. Chem., 265(24), 14599-14605 (1990).
- Schumacher, T.N., et al, Science, 271, 1854-1857 (1996).
- Tang, A., et al, Nature, 361, 82-85 (1993).
- Chui, N.H, et al, Clin. Chem., 45, 1954-1959 (1999).
- NHS LC biotin biotinylates, amino acids, peptides or proteins under mild conditions
- Penetrates cell membranes and can be used to label inside cells
- Soluble in DMSO and DMF. Dissolve in DMSO or DMF prior to dilution in aqueous systems
- NHS LC biotin is used to biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resin
- Forms stable amide bonds therefore the reaction is not reversable