BIOTIN HPDP

(Biotinylation Reagent)

B106

N-[6-(Biotinamido)hexyl]-3-(2-pyridyldithio)propionamide

Formula Weight: f.w.: 539.8 daltons

HPDP biotin is a pyridyl dithiol biotin compound for labeling protein cysteines and other molecules that contain sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in near neutral buffers to form reversible disulfide bonds. Biotinylation occurs between pH 6-9. The pyridine-2-thione leaving group can be measured at 343nm to determine the degree of biotinylation.

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Pricing & Ordering

Quantity Price Action
50mgm + $256.00
100mgm + $483.00
Product Documents
Certificate of Analysis


Spacer arm 29.2 angstroms. Sulfhydryl reactive biotinylating reagent. Biotinylation occurs between pH 6-9. Pyridyl dithiol reacts with sulfhydryl groups to form a stable sulfhydryl bond. The pyridine-2-thione leaving group can be measured at 343nm to determine the degree of biotinylation. Purity >94% by HPLC.

    

  1. Ghebrihiwet, B., et al, J. Immunol. Methods, 110, 251-260 (1988). 
    2. 

  2. Ishmael, F.T., et al, J. Biol. Chem., 276, 25236-25242 (2001). 
    3. 

  3. Slatin, S.L., et al, Proc. Natl. Acad. Sci. USA, 99, 1286-1291 (2002).
    4. 

    

  • Biotin HPDP biotinylates antibodies or other proteins for use in protein methods 
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  • Thiol reactive - Biotin HPDP reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C) 
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  • Pyridyldithiol activated perform reactions at pH 6.5 to 7.5 in buffers such as PBS 
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  • The reaction is reversible and forms disulfide bonds, which can be cleaved using DTT, TCEP, DTBA or other reducing agent 
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  • Must be dissolved in DMSO or DMF before further dilution in aqueous buffers 
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  • Medium length spacer arm is 29.2 angstroms
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