APO 12

Alkylphosphine oxides ( APO ) are a family of non ionic detergents with a wide range of versatile uses. The detergents are pure crystalline solids, soluble in water and the solutions are stable over long periods. Ideally the alkyl phosphine detergents have low CMC values, are UV transparent and P31 NMR detectable. Alkyl phosphine oxide detergents have been used for purification of high purity DNA without the need to use resins or phenol/chloroform. APO 10 has been used in the two phase separation and purification of proteins. CMC (H2O) ~ 4.66 mM (0.10%) Aggregation Number (H2O) ~ 31 Purity 99% pure by HPLC analysis. pH (1% solution) 5-8 Solubility in water at 0-5°C 20% Conductance (10% solution) < 100 µS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1

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Kresheck, G.C., and Huang J., Chem Phys. Lipids, 76, 193, (1995 ).

Novel non ionic detergent useful in two phase separation and purification of proteins 
APO 12 gives excellent yields of high quality DNA 
Purification of plasmid DNA using APO 12 does not require the use of chloroform / phenol or resins