DSP

(Protein Crosslinker homobifunctional)

CL102

Dithiobis(succinimidylpropionate)

CAS#: [ 57757-57-0 ]

Formula Weight: f.w.: 404.4 daltons

DSP (Lomant\'s Reagent) is an amine reactive crosslinker having NHS ester reactive ends and a cleavable disulfide bond in its spacer arm.

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Pricing & Ordering

Quantity Price Action
1gm $282.00
5gm + $1,333.00
Product Documents
Certificate of Analysis


Spacer arm 12.0 angstroms. Cleaveable homobifunctional reagent. A thiol cleaveable crosslinking reagent. To reduce the disulfide bond use 50mmoles DTT, 100mmoles 2-mercaptoethanol or 1% sodium borohydride.

    

  1. Adir, N. and Ohad, I. (1986). Biochim. Biophys. Acta 850(2), 264-274. 
    2. 

  2. Appenzeller-Herzog, C., et al. (2005) Carbohydrate- and Conformation-dependent Cargo Capture for ER-Exit Mol. Biol. Cell. 16, 1258-1267. 
    3. 

  3. Joshi, S. and Burrows, R. (1990). AT synthase complex from bovine heart mitochondria. J. Biol. Chem. 265, 14518-14525. 
    4. 

  4. Leduc, M., et al. (1989). Microbiol. Lett. 51(1), 11-14. 
    5. 

  5. Mattson, G., et al. (1993). A practical approach to crosslinking. Molecular Biology Reports 17, 167-183. 
    6. 

  6. Partis, M.D., et al. (1983). Crosslinking of proteins by omega-maleimido alkanoyl N-hydroxysuccinimide esters. J. Protein. Chem. 2, 263-277. 
    7. 

  7. Xiang, C.C., et al. (2004) Using DSP, a reversible crosslinker, to fix tissue sections for immunostaining, microdissection and expression profiling. Nucleic Acids Res.32, e185.
    8. 

    

  • Amine reactive NHS ester reacts rapidly with any primary amine containing molecule 
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  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5 
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  • Spacer arm is also cleaved with 5% ?-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes 
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  • Cleavable crosslinker allows separation of crosslinked products 
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  • DSP crosslinker is membrane permeable, so intracellular crosslinking can be done
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