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DSP(Protein Crosslinker homobifunctional) CL102 Dithiobis(succinimidylpropionate)[ 57757-57-0 ] f.w.: 404.4 daltons |
DSP (Lomant\'s Reagent) is an amine reactive crosslinker having NHS ester reactive ends and a cleavable disulfide bond in its spacer arm.
| Quantity | Price | |
|---|---|---|
| 1gm | $282.00 |  |
| 5gm + | $1333.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 12.0 angstroms. Cleaveable homobifunctional reagent. A thiol cleaveable crosslinking reagent. To reduce the disulfide bond use 50mmoles DTT, 100mmoles 2-mercaptoethanol or 1% sodium borohydride.
- Adir, N. and Ohad, I. (1986). Biochim. Biophys. Acta 850(2), 264-274.
- Appenzeller-Herzog, C., et al. (2005) Carbohydrate- and Conformation-dependent Cargo Capture for ER-Exit Mol. Biol. Cell. 16, 1258-1267.
- Joshi, S. and Burrows, R. (1990). AT synthase complex from bovine heart mitochondria. J. Biol. Chem. 265, 14518-14525.
- Leduc, M., et al. (1989). Microbiol. Lett. 51(1), 11-14.
- Mattson, G., et al. (1993). A practical approach to crosslinking. Molecular Biology Reports 17, 167-183.
- Partis, M.D., et al. (1983). Crosslinking of proteins by omega-maleimido alkanoyl N-hydroxysuccinimide esters. J. Protein. Chem. 2, 263-277.
- Xiang, C.C., et al. (2004) Using DSP, a reversible crosslinker, to fix tissue sections for immunostaining, microdissection and expression profiling. Nucleic Acids Res.32, e185.
- Amine reactive NHS ester reacts rapidly with any primary amine containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also cleaved with 5% ?-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
- Cleavable crosslinker allows separation of crosslinked products
- DSP crosslinker is membrane permeable, so intracellular crosslinking can be done