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DTSSP(Protein Crosslinker homobifunctional) CL103 3,3'-Dithiobis(sulfosuccinimidylpropionate)[ 81069-02-5 ] f.w.: 608.5 daltons |
DTSSP is a water soluble crosslinker that contains amine reactive NHS ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents such as DTT, TCEP or DTBA.
| Quantity | Price | |
|---|---|---|
| 50mgm + | $127.00 |  |
| 100mgm + | $240.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 12.0 angstroms. Homobifunctional reagent. Thiol cleaveable crosslinker. The disulfide bond can be cleaved with 50mmoles DTT, 100mmoles 2-mercaptoethanol, or 1% sodium borohydride. Has been used to immobilize proteins onto supports containing amino groups. Purity >95% by HPLC.
- Back, J. W., et al. (2002). Protein Sci. 11, 2471-2478.
- Bennett, K.L., et al. (2000). Protein Sci. 9, 1503-1518.
- Cheng, S.K., et al. (2004) PloS Biology.2, e30.
- Paine-Saunders, S., et al. (2002) Heparan Sulfate Proteoglycans Retain Noggin at the Cell Surface. A Potential Mechanism for Shaping Bone Morphogenetic Protein Gradients.J. Biol. Chem.277, 2089-2096.
- Liliana Schaefer, L., et al. (2005) The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages. J. Clin. Invest.115, 2223-2233.
•Amine reactive sulfo NHS ester reacts rapidly with any primary amine containing molecule
•Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
•Spacer arm is also cleaved with 5% ?-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
•Cleavable crosslinker allows separation of crosslinked products
•DTSSP crosslinker is not membrane permeable, so it is used for cell surface crosslinking