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SULFO NHS SS BIOTIN(Biotinylation Reagent) B105 Sulfosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionatef.w.: 606.7 daltons |
Sulfo NHS SS Biotin is a thiol cleavable amine reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding. This reagent is water soluble, enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents.
| Quantity | Price | |
|---|---|---|
| 50mgm + | $168.00 |  |
| 100mgm + | $317.00 | |
| Download***Certificate of Analysis*** | ||
Spacer arm 24.3 angstroms. Cleavable biotinylation reagent. A water soluble biotinylation reagent that is reversible. Useful for extracting non covalently associated proteins from complex physiological mixtures under mild isolation conditions. Soluble in water.
- Shimkus, M., et al, Proc. Natl. Acad. Sci., 82, 2593-2597 (1985).
- Duhamel, R.C., Johnson, D.A., J. Histochem. Cytochem., 33, 711-714 (1985).
- Gottardi, C.J., et al, Am. J. Physiol., 268(2), F285-F295 (1995).
- Soukup, G.A., et al, Bioconjugate Chem., 6, 135-138 (1995)
- Daniels, G.M., Amara,S.G., Methods Enzymol., 296, 307-318 (1998).
- Huh, K.H., Wenthold, R.J., J. Biol. Chem., 274, 151-157 (1999).
- Trotti, D., et al, J. Biol. Chem., 276, 576-582 (2001).
- Protein labeling biotinylate antibodies to facilitate immobilization, purification or detection
- Sulfo NHS SS Biotin biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine reactive It reacts with primary amines (-NH2), such as the side chain of lysines (K) or the amino termini of polypeptides
- A cleavable disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT, TCEP or DTBA only a small sulfhydryl group remains attached to the molecule
- The charged sulfo NHS group increases reagent water solubility compared to ordinary NHS ester compounds
- Sulfo NHS SS Biotin has a medium length spacer arm (total length added to target) of 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain